Muscatatuck National Wildlife Refuge is located in south central Indiana. It includes 7,724 acres near Seymour and a 78-acre parcel, known as the Restle Unit, near Bloomington. Muscatatuck's mission is simple: to restore, preserve, and manage a mix of forest, wetland, and grassland habitat for fish, wildlife, and people. Special management emphasis is given to waterfowl, other migratory birds, and endangered species. Nine miles of refuge roads that are open sunrise to sunset seven days/week attract approximately 185,000 visitors to the refuge each year. Wildlife-viewing opportunities are excellent at Muscatatuck, and the refuge is known as an exceptionally fine bird watching site.

The refuge is located in south-central Indiana on U.S. Highway 50, just three miles east of the I-65/U.S. 50 interchange at Seymour, IN. Muscatatuck is approximately an hour's drive from Louisville, Kentucky, and Indianapolis, Indiana, and is approximately 86 miles from Cincinnati, Ohio. The main entrance on U.S. Highway 50 is marked with large brown signs.