Minnesota Valley National Wildlife Refuge is located within the urban and suburban areas of Minneapolis and St. Paul. It is a green belt of large marsh areas bordered by office buildings, highways, residential areas, and grain terminals. The refuge is comprised of eight linear units totaling approximately 12,500 acres, spanning 34 miles of the Minnesota River. The focal point of the refuge is the visitor center, which features 8,000 square feet of exhibit space, a 125-seat auditorium, two multi-purpose classrooms, a bookstore, an art gallery, and an observation deck. Environmental education and interpretation are conducted from this facility. Recreational activities include hiking, biking, cross-country skiing, hunting, and fishing.

The Refuge Office and Visitor Center are located just off of 34th Avenue in Bloomington, Minnesota, across the street from the Minneapolis/St.Paul Airport Hilton Hotel. From I-494, visitors should take the 34th Avenue exit south to East 80th Street. Turn left on East 80th Street and proceed east for 1/4 mile to the Visitor Center entrance which is on the right.