James River National Wildlife Refuge (NWR) is one of four refuges that comprise the Eastern Virginia Rivers National Wildlife Refuge Complex. The Refuge encompasses 4,200 acres of forest and wetland habitats along the James River, bordered by Powells Creek to the west, and the historic Flowerdew Hundred Plantation to the east. Located in Prince George County, Virginia, the refuge is 8 miles southeast of the City of Hopewell and thirty miles southeast of the City of Richmond.

The Refuge was created in 1991 to protect nesting and roosting habitat for the threatened American bald eagle. A secondary objective is to provide an opportunity to view wildlife in its natural environment, so that the public may better appreciate the refuge's role in conservation of wildlife resources.

Public access is only available for pre-registered visitors and participants of refuge sponsored events. 4550 Flowerdew Hundred Road Hopewell, VA 23860 Take Route 10 south/east toward Hopewell. After passing through Hopewell, proceed another 8 miles to Route 639. Take a left and look for the Refuge entrance sign.